FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R21_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R21_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42191329
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1169683027.723303051202773No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1124064926.64208325838705No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT806460919.114375373195756No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC19970574.733335136231428No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA19211054.553317104564305No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT13715993.2509025728959617No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4590711.0880695414927555No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC2865860.6792533129259806No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA2385050.5652938782753205No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1685340.3994517451678282No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT1503590.3563741734705726No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1113410.2638954558648769No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1066360.25274387540624754No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA897990.21283757143559048No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC785410.18615436361343346No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC711070.168534629473274No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA640780.15187480820999974No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGAACCCCTACCAACT583760.13836018296555674No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAAC535060.12681752689041864No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGTACCCCTACCAAC488050.1156754270527956No Hit
TCGATCGTTACATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT485570.11508762855040665No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGAACCCCTACCAAC459290.1088588605492849No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGAACCCCTACCAACT425260.10079322222819764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)