FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R20_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R20_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41669557
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1144841527.47429016343994No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1088474226.12156879901555No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT829244419.90048514314659No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC20856455.005200799230959No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA19807884.753561454948993No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT15044753.6104895475610648No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC3654670.8770599601046875No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC2631760.6315785886564621No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA2216460.5319135022241777No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1327250.3185179050499625No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1093920.26252258933302314No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1014000.24334311977446751No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC822640.19741990537600387No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA792540.19019640645567698No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT791530.18995402326931385No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC581830.1396295141798604No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGAACCCCTACCAACT569060.13656492676416024No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA556430.13353393701785696No Hit
TCGATCGTTACATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT477030.11447925880277536No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGTACCCCTACCAAC472940.11349772688968111No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGAACCCCTACCAAC451770.10841727931016881No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTATCAAC418270.10037783699020367No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGAACCCCTACCAACT416850.10003706062917828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)