FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R19_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R19_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55635124
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1523794627.38907528991937No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1427082925.650754368768908No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1105744619.874937278831265No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC28050685.041901227720818No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA26173914.704565770357589No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT20234353.6369740094405114No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4959020.8913469843259448No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC3609140.648716088059766No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA3260850.5861135494188887No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA2356880.4236316611786468No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1794470.32254264410375No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1421780.2555543868294425No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA1355250.24359611385066743No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1167520.20985304175829642No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT1012560.18200013358467573No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA853280.1533707375218576No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAA796340.14313619576007416No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC791510.1422680391617353No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGAACCCCTACCAACT768960.13821484427715125No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGTACCCCTACCAAC658140.11829577300843258No Hit
TCGATCGTTACATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT629180.11309042826973838No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAA601290.1080774080776741No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGAACCCCTACCAAC586560.10542980006659101No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAAC565250.10159948596501735No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGAACCCCTACCAACT564480.10146108418847058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)