FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R18_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R18_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74793384
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1136363015.193362557308546No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1131827415.13272082996004No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC980989613.115994323776018No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT851382111.383120464237853No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC779952110.428089468448173No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA56482297.55177623732067No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA20186152.698921872554931No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC20105022.6880746564428746No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC17235022.3043508768101737No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT15044612.011489411951196No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC13590681.8170965496092544No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA9656991.2911556455314281No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC7250380.9693878806178899No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4838870.6469649775440031No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC2813510.37617097255554044No Hit
ACGATCGATAGGTAAGGTCATGTGGAAAGGACGAAACACCGAACCCCTAC2588960.3461482635950795No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA2422490.323890947359729No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC2356050.3150078087120647No Hit
CGATCGATACAGGTATTCTAGTGGAAAGGACGAAACACCGAACCCCTACC2193370.293257221788494No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA1831910.244929417821234No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1387670.18553378999404546No Hit
CCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1172680.15678926895459094No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCA1149050.15362989860172657No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1119040.1496175116237554No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGTACCCCTAC1083250.1448323290199037No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA854980.11431224986423934No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT838200.11206873591920911No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC787180.10524727695166193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)