FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R17_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R17_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101296047
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1469346114.505463377065444No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1440887514.22451855401623No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1438603314.201968809306054No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1110364910.96158174859479No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1061094310.475179747142551No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA83239698.217466768471231No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC25855692.5524875615333733No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA25300472.497675945834293No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC25236382.4913489467165486No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC19392731.914460689665412No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT18593811.8355908794742999No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA14183801.4002323308825664No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC10609881.0474130347850592No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC4916310.4853407556960243No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4880710.4818263046335856No Hit
ACGATCGATAGGTAAGGTCATGTGGAAAGGACGAAACACCGAACCCCTAC3772300.37240347592241185No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC3443400.3399342918090377No Hit
CGATCGATACAGGTATTCTAGTGGAAAGGACGAAACACCGAACCCCTACC3125750.3085757137196084No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA3027530.298879382726554No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA2685650.2651288060628862No Hit
CCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC2182660.21547336393097352No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1875960.18519577570485055No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCA1644360.16233209969190604No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGTACCCCTAC1587800.15674846620618868No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1369730.13522047903804182No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT1227280.12115773876151356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)