FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R15_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R15_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99214678
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1518813315.308352862869745No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1479480414.911910513885859No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1332088213.426321859352303No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1105783211.145358955859333No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1054675910.630240618227878No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA75811317.64113854202097No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC26948212.716151535562107No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA26181262.6388494653986583No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC23127462.331052266278584No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT19508251.9662665235883745No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC18193431.8337437934334677No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA12856831.2958596710861672No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC9958571.0037395878057478No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4785010.48228851783402454No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC3554900.3583038388735183No Hit
ACGATCGATAGGTAAGGTCATGTGGAAAGGACGAAACACCGAACCCCTAC3527930.3555854910903405No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA3108180.3132782429632035No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC3098150.3122673038358296No Hit
CGATCGATACAGGTATTCTAGTGGAAAGGACGAAACACCGAACCCCTACC2934540.29577680028352255No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA2322410.23407927605227927No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1794540.18087444682328152No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCA1529500.15416065755915673No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGTACCCCTAC1485150.14969055284340085No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1451200.1462686801241244No Hit
CCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1287300.12974894702576165No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA1109680.1118463540243511No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT1105040.1113786812874603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)