FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R14_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R14_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85143727
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1332956915.6553741181661No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1318135215.481295527502573No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1096484412.878040915451116No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT980884611.520339014523055No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC869788610.215533553047308No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA62617577.354337448723615No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC24060512.825869955164166No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA23856022.801852918653655No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC19424652.2813953164159706No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT17663472.0745474296656052No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC15280091.7946231082884119No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA10833471.2723744169667368No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC7442490.8741090227351688No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4072950.47836172358299517No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC3055870.35890723928493284No Hit
ACGATCGATAGGTAAGGTCATGTGGAAAGGACGAAACACCGAACCCCTAC2860960.33601535906456154No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA2673040.3139444436112129No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC2443870.28702877899624946No Hit
CGATCGATACAGGTATTCTAGTGGAAAGGACGAAACACCGAACCCCTACC2399790.2818516506800319No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA1805880.21209783311458755No Hit
CCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1624150.19075392365664237No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1570640.18446925631996353No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCA1240020.14563844497904116No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1214320.14262001944077454No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGTACCCCTAC1110160.1303865873759555No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA926760.10884653898225526No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT925750.10872791603308604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)