FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ox_60_240_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ox_60_240_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8005124
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT7750.012.9119391
CGCGTAT5155.456968E-1212.7303851
CGAAAAC44800.011.78443051
CCGGCAT15850.011.7560021
CGGAAAT30100.010.3174221
CGCGAAC5058.77335E-810.249321
CGAAAAT43150.010.235861
CGACAAT30600.010.0360721
CGGCAAT20050.09.9817991
CGACACT17050.09.9166931
CGGCTAT11000.09.724431
CGGCACT15650.09.7013811
CTCGAAT30800.09.6348051
CGCGGAT11650.09.4780551
CGGGAAT19750.09.4345651
GTCGAAT21450.09.3303051
CCCTACA12300.09.2566210
CGGACAT12800.09.1656681
GAAAAAT67350.09.11963751
CGGGCAT9900.09.0621931
Produced by FastQC (version 0.11.2)