FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_4bw_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_4bw_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14530984
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG271870.18709675820990512RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT96950.057.2567742
CGTATGC105600.054.4615944
TCGTATG106800.053.72044443
ACGTCTG115200.051.8679515
TATGCCG110700.051.6088146
CACGTCT122400.048.95799314
GTATGCC121350.047.392745
CAGTCAC125400.047.1272927
AGTCACC128450.046.0616728
ATGCCGT127500.044.8353447
GCCGTCT122450.044.73978449
AGATCTC126800.044.1579538
ACACGTC136900.043.9253813
CCAGTCA140700.042.39489426
GTCTGAA143800.041.74399617
TCTCGTA134800.041.3072541
CCGTCTT133450.041.07811450
GCTTGAA130350.040.54631459
CTGAACT149900.040.022419
TCGGAAG152000.039.2487033
Produced by FastQC (version 0.11.2)