FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_4bw_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_4bw_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12858239
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA373450.2904363497987555TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT113950.059.76418344
CGTATGC120500.056.9734546
TATGCCG122050.056.44798748
TCGTATG118300.056.39988745
ACGTCTG151400.056.32795715
AGTCACA153150.055.393228
ACATTCC151950.055.33115832
GTCACAT155150.054.4345429
CACGTCT158800.053.83324414
CAGTCAC157550.053.64849527
CCTTTAT158350.052.96330637
TTATCTC138700.052.23372340
GTATGCC137800.050.52175547
CTTTATC163400.050.20723338
ACACGTC172250.049.7518213
TATCTCG145250.049.54554741
TCTCGTA138350.049.2987343
GTCTGAA173250.049.2241617
CCAGTCA173300.048.9521226
ATGCCGT136600.048.79379349
Produced by FastQC (version 0.11.2)