FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_4bw_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_4bw_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14313603
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA154990.10828161155510602TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT67400.051.54383529
AGTCACG74350.046.9112928
ACGTCTG75300.046.82341415
CTCGTAT56700.045.57409744
CGTATGC58000.045.20691746
GCCTTAT77100.044.56655537
TCGTATG59050.043.9356745
CACGTCT81700.043.3669214
TATGCCG63600.042.14801448
TCACGTG84450.041.38237830
CCTTATC83850.039.7037438
CAGTCAC93600.037.3735827
CTTATCT78150.037.30262839
GTATGCC72850.037.12797547
CACGTGG96100.036.2579831
ACACGTC100150.035.4482113
ACGTGGC99250.035.31578432
TTATCTC80700.034.58527840
TATCTCG81200.034.03241341
GTCTGAA106150.033.47482717
Produced by FastQC (version 0.11.2)