FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_4bw_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_4bw_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14212923
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA319130.22453509387196424TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT96750.056.48244529
CTCGTAT92750.055.6832844
AGTCACG99000.055.3385428
TCGTATG99050.054.8233645
CGTATGC100700.053.7882146
TATGCCG100950.053.1758148
ACGTCTG107550.051.67593415
CACGTGA110100.049.79043231
CACGTCT112100.049.73269714
TCACGTG111500.049.25808330
TGAAACG111300.048.97463635
GAAACGA111300.048.54051236
GTATGCC113650.047.56750547
CAGTCAC115950.047.33836727
ATGCCGT116800.046.2843349
GCCGTCT118200.044.45173351
GTCTGAA131700.042.27863717
GCTTGAA119950.042.22119561
TCTCGTA121800.042.14757543
CCGTCTT126700.041.63279752
Produced by FastQC (version 0.11.2)