FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_4bs_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_4bs_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15228206
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA190880.12534634742923756No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTGAATTGCAGAATCCCGTGAACCATCG163650.10746505530592376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34950.045.80243
CTCGTAT34100.045.3249442
TATGCCG35100.045.11466646
GTATGCC42000.038.03141445
GTCACAT43350.037.881529
ACGTCTG44850.037.2298115
AGTCACA45200.036.25471528
CAGTCAC45500.036.16743527
ACACGTC49900.034.2242513
ATGCCGT48400.032.859847
CGTATGC49550.032.16699644
CACGTCT53050.031.86555114
GTCTGAA54050.030.89271717
CACGATC53600.028.8994436
TCACGAT65950.027.3587535
ATCACGA66900.027.17651734
GCCGTCT58100.026.7798649
GCAACGG46300.025.8593563
CCGTCTT61500.025.52373350
AGCACAC71550.023.91819810
Produced by FastQC (version 0.11.2)