FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_3bw_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_3bw_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10358777
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG116600.053.8491315
ACCGGAT116150.052.21723635
CACGTCT123250.050.99965714
CAGTCAC125450.049.94100627
AGTCACC130150.048.0049928
CGTCTGA130800.048.0028916
CCAGTCA132800.047.0210826
ACACGTC135450.046.50889213
CGGATCG119850.046.31590337
GATCGGG115650.045.6412939
CACACGT138350.045.6107912
TCGGAAG137600.045.4865883
GTCTGAA139400.045.18993817
ATCGGGG110700.044.4098540
ATCGGAA141800.044.2609752
CCGGATC130850.043.23986436
TCCAGTC146850.042.61606225
TCTGAAC149250.042.34644318
GCACACG151050.041.80118611
AGCACAC156850.040.52021410
Produced by FastQC (version 0.11.2)