FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_3bw_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_3bw_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11145973
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42500.049.67889442
ACGTCTG51000.047.08088715
TCGTATG49200.045.50813343
CGTATGC51600.043.12423344
TATGCCG51700.043.10793746
CACGTCT57700.041.85330214
CAGTCAC61200.039.4031827
CTAATCT56400.038.41386837
AGTCACC63750.037.3400128
GTATGCC60750.037.0834645
TAATCTC62550.034.47150438
ATGCCGT64650.034.09916347
ACACGTC71650.034.09133513
ACTAATC66150.033.1691836
CCAGTCA72850.033.00720626
GTCTGAA76000.032.00214817
GCCGTCT65750.031.4820549
CCGTCTT68600.030.42570750
TCGGAAG81050.029.8427433
TCTCGTA71450.029.79136541
Produced by FastQC (version 0.11.2)