FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_3bw_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_3bw_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680239
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC42350.047.65480838
CTCGTAT35000.045.5391644
TACGTAA47550.043.9669536
TCGTATG37650.041.5093645
CCGTACG51500.040.46091533
TATGCCG38350.040.3017448
ACGTAAT52900.039.6508237
CAGTCAC57450.037.0513927
ACGTCTG57700.036.6506815
TAATCTC50450.035.4218440
CGTATGC45400.034.34765646
CACGTCT62000.034.33148614
AGTCACC62350.034.1947728
GTATGCC47250.033.2218747
ACCGTAC70950.030.68182432
CACCGTA70600.029.75913231
ACACGTC73950.029.06448713
ATGCCGT53800.028.66374649
GTCTGAA76350.028.19561217
CGTACGT76300.027.62629334
Produced by FastQC (version 0.11.2)