FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_3bw_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_3bw_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13114227
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG39550.052.59972833
GTCACGT42300.049.34324329
TTTCGGA44500.046.90415635
AGTCACG45150.046.53419528
TTCGGAA45150.046.15249336
ACGTCTG47750.044.505315
ACGTTTC48200.043.30363532
CACGTTT51500.040.46184531
CACGTCT53400.040.0551314
CAGTCAC56200.037.75327
TCGGAAT57000.036.43630237
ACACGTC63050.034.19997813
GTCTGAA66600.032.3231117
CCAGTCA74100.029.14531726
AATCGCG14400.028.2700941
AGCACAC83450.026.13102310
GTTTCGG80850.026.07207134
CTGAACT83750.025.62181919
CACACGT84950.025.30319812
TCTGAAC87450.024.85326618
Produced by FastQC (version 0.11.2)