FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir640_3bs_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir640_3bs_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15331992
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29200.043.7151942
ACGTCTG62150.043.63026415
CAGTCAC69000.038.84911727
CACCGAT69450.038.59765231
TCGTATG31050.038.443743
CACGTCT71950.038.1191114
ACACGTC73150.037.4009213
TATGCCG36300.036.97047846
AGTCACC75600.035.5945528
GTCTGAA81800.033.61321317
CGATGTA80400.033.0401934
TGTATCG26750.031.21030437
CGTATGC41850.029.6769444
ACCGATG90850.029.46778332
GATGTAT90400.029.4615335
TGTATCT59650.029.38053537
CACACGT93600.029.23050512
CCGATGT91400.028.91281733
CCAGTCA92700.028.87960426
GTATGCC47900.028.80955745
Produced by FastQC (version 0.11.2)