FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12863995
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG233790.18173980944488863TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG189000.061.5139132
TCGTATG91800.059.97941243
CTCGTAT93200.059.8187642
AGTCACT197250.059.0989528
GTCACTT198700.058.61559729
TATGCCG101500.057.54482346
ACGTCTG204150.057.5246315
CAGTCAC202750.057.4109227
TTAGGCA204600.056.77311734
CACGTCT213150.055.14449714
ACACGTC216450.054.51181413
CGTATGC105800.054.2273744
GTCTGAA218250.053.93409717
TCACTTA220350.053.2008930
CACTTAG223300.052.59055331
GTATGCC114850.052.0572545
CCAGTCA224050.052.0145726
TAGGCAT222600.051.4383835
ATGCCGT107450.051.2438647
CTTAGGC227500.051.07353233
Produced by FastQC (version 0.11.2)