FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8138240
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG84260.10353589965397925No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA81740.10043940704624096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT28600.056.57346339
ACGTCTG40900.054.06771515
CTCGTAT25300.052.90814244
TCCGTAT41200.052.08337437
TCGTATG24950.051.85245545
TATGCCG27450.050.90009748
AGTCACA43500.050.5188528
CACGTCT44250.050.36426514
TTCCGTA42900.050.2607236
CCGTATC41600.049.5925538
CAGTCAC44100.049.51860427
GTCACAG44600.049.3502229
ACACGTC46000.048.37472513
CGTATCG10000.045.5386239
GTATGCC31850.044.95154647
CGTATGC30450.044.86591346
GTCTGAA50000.044.36538317
ATGCCGT31250.041.84007649
AGCACAC54600.041.4556610
TCACAGT53250.040.88020330
Produced by FastQC (version 0.11.2)