FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309113
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA177000.15651094829453024TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT56200.055.98515344
TCGTATG60700.054.10789545
TATGCCG59250.053.62726248
ACGTCTG68050.050.2907715
CGTATGC65000.049.73253346
GTATGCC65700.049.09763747
CACAGTC70150.048.4410231
AGTCACA70700.048.4061828
CACGTCT72000.047.62780414
GTCACAG72850.046.740829
ACAGTCA73750.046.2633532
CAGTCAC74250.046.09181227
ATGCCGT68200.045.9314449
ACACGTC77800.044.34457413
GTCTGAA82150.041.91078617
GCCGTCT73250.040.88092451
CAATCTC78950.040.2455640
GTCAACA84750.040.05513835
TCACAGT85150.039.90801630
CCGTCTT77000.039.2930352
Produced by FastQC (version 0.11.2)