FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13502824
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23900.039.84024442
TCGTATG23750.037.9128243
AGTCACG38350.037.8729528
ACGTCTG43000.034.659815
TATGCCG27200.032.089746
CAGTCAC47750.030.6339727
GGTAGCA48300.030.07096534
CACGTCT49800.030.06580214
TAGCATC47750.028.8277236
ACACGTC53700.028.33228113
GTCACGG57900.025.68095229
GTCTGAA58600.025.6680717
CGTATGC34650.025.28965644
GTATGCC37550.023.52036345
ATGCCGT37150.022.19489547
GTAGCAT75500.022.11613535
CCAGTCA67700.021.86147326
AGCACAC72650.020.89608210
CACACGT75550.020.13890512
TATCTCG42550.020.1110025
Produced by FastQC (version 0.11.2)