FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14962223
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT47100.047.0243444
AGTCACG51500.044.8819628
TCGTATG51750.043.5321445
TATGCCG52750.042.24893648
GTCACGA55000.042.21401229
CACGAGT55600.041.69627831
ACGTCTG57700.041.07654615
CGTATGC56800.039.54028746
TCACGAG58850.039.39359330
CACGTCT63950.037.11572614
ACGAGTG64600.036.42123432
CAGTCAC64950.035.80016327
GTATGCC63850.035.55254747
ATGCCGT67900.032.7712349
ACACGTC74500.031.81492213
GTCTGAA76650.031.01078817
TGGATAT78400.029.7024337
GCCGTCT77350.027.69699551
CCAGTCA85200.027.4937826
CCGTCTT80600.026.53737852
Produced by FastQC (version 0.11.2)