FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14410880
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG60650.042.88956515
TCGTATG24100.042.37265843
CAGTCAC60950.042.2825327
CTCGTAT20000.041.0542442
TATGCCG28700.040.87046446
AGTCACC64600.040.10725828
CACGTCT65500.039.8192514
ACACGTC70050.037.23439413
CGTCCAT66000.035.75400535
GTCTGAA72950.035.70505517
GTATGCC37900.031.22248645
CGTATGC36850.029.86522544
AGCACAC89900.029.43689210
ATGCCGT36650.028.99264347
CCAGTCA92300.028.18257726
CTGAACT94100.027.9733519
GAGCACA98350.027.6092839
GAACTCC96750.027.17149521
GTCGTAT8600.026.07509842
CTCCAGT100650.025.98145724
Produced by FastQC (version 0.11.2)