FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bw_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bw_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15270145
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG89200.044.2070215
CAGTCAC92800.041.7861627
AGTCACA93900.041.4802228
GTCACAT95200.040.8775529
CACGTCT98300.040.36027514
ACACGTC104400.038.00318513
CACATGT103050.037.8975531
GTCTGAA108400.036.59929317
AATCGCG21950.036.3067441
ATGTCAG108150.035.91867434
GTCAGAA113750.033.90762736
CCAGTCA114850.033.8237326
CTCGTAT26950.032.89900644
CATGTCA118900.032.84528433
TGTCAGA118700.032.7262435
CACACGT123400.032.2642712
TCACATG124400.031.36579930
AGCACAC128450.031.26572810
TCTGAAC134300.029.84930818
CTCCAGT134300.029.33583824
Produced by FastQC (version 0.11.2)