FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bs_17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bs_17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13368897
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA144560.10813158333107062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43100.045.62435515
CTTATCG22750.043.97649437
AGTCACT44500.043.41487528
CAGTCAC48600.039.89412727
ACACGTC49550.039.75617613
CACGTCT49800.039.69425614
GTCTGAA56350.034.83526617
CTCGTAT16400.032.39546242
TTATCGG17450.029.85283338
TATGCCG19950.028.36023746
TTATCGC16800.028.13295238
AGCACAC70650.028.08020810
TCGTATG15950.027.2532843
CTTATCT32950.026.17511637
CCAGTCA74550.026.05381226
GAGCACA79750.025.7847829
GTCACTA77500.025.77413629
CTGAACT77050.025.70069719
TATCGCG19250.025.26944239
GAACTCC82350.024.13067221
Produced by FastQC (version 0.11.2)