FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_4bs_17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_4bs_17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12715936
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA127780.10048808046847672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44600.053.52892343
CTCGTAT42650.052.90247342
TATGCCG46550.050.69346246
AGTCACG48550.049.67048328
GTCACGA53600.045.0550729
ACGTCTG54550.045.02881615
CGTATGC54650.043.43239644
GTATGCC54750.043.1640345
CAGTCAC57450.042.1558227
ATGCCGT56800.041.6667547
ACACGTC59500.041.40080313
CACGTCT61600.040.21154414
GTCTGAA65350.037.79878617
CACGATC65500.037.02736331
GCCGTCT65750.035.31246649
CCGTCTT69600.033.3095550
AGATCTC69500.032.91085438
TCAGATC69000.032.8992136
AGCACAC77750.031.95118310
TCACGAT76150.031.71308930
Produced by FastQC (version 0.11.2)