FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13127735
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG211020.16074364694290372TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT73250.059.90819542
TCGTATG79200.059.28429843
TATGCCG80000.057.613846
ACTGACC85600.057.14929632
AGTCACT89900.055.0302128
ACGTCTG91700.054.70057715
CAGTCAC92900.053.327427
GTATGCC88900.052.03997445
ATGCCGT87950.051.69949747
CACGTCT97700.051.58923314
CGTATGC92200.050.3268544
CTGACCA98000.050.0235833
ACACGTC100900.049.9569113
GTCTGAA102250.049.0234717
ACCAATC93200.047.8986736
CAATCTC94550.047.3605438
CCGTCTT94400.044.9874350
GCCGTCT94300.044.8889749
AGCACAC115300.043.9320310
CCAATCT104800.042.7942637
Produced by FastQC (version 0.11.2)