FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14362134
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT18900.048.37251742
TCGTATG31050.047.44412243
TATGCCG37300.043.93401346
ACGTCTG70950.043.7139815
AGTCACA70500.043.50365428
CAGTCAC71400.043.0031627
CACAGTG71850.042.7339833
ACACAGT72450.042.47545232
GTCACAC74700.041.61187729
CACGTCT76900.040.64573714
CACACAG79350.038.86889631
CTCGTAT19300.037.1809642
GTCTGAA83900.037.17140617
ACACGTC85650.036.61497513
CGTATGC45350.035.90714344
GTATGCC47800.035.36586845
ACAGTGA87000.035.2528434
AGCACAC94200.033.62383310
CCAGTCA94500.032.49150526
ATGCCGT47850.031.29110347
Produced by FastQC (version 0.11.2)