FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13328236
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG212950.15977358144018458TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT82700.056.2336942
GTCACGC93450.053.05012529
TCGTATG88750.052.82789643
AGTCACG94300.052.60872728
TATGCCG89650.051.45055846
CGTATGC90000.051.4423344
GTATGCC91550.050.75979245
ACGTCTG100600.050.3776215
CCAATAT101400.049.06135635
CAGTCAC104000.047.9675127
TCACGCC104000.047.76811630
GCCAATA104800.047.70028334
ACGCCAA104800.047.50240732
CACGTCT106950.047.45121814
CGCCAAT105000.047.41192633
ATGCCGT98300.046.25612347
CACGCCA109850.045.3184731
ACACGTC115300.044.1956713
GCCGTCT98150.043.8310549
CAATATC112450.043.6573836
Produced by FastQC (version 0.11.2)