FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10970645
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG257660.23486312792000835TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT83700.060.46693842
TCGTATG102550.060.15159643
TATGCCG100950.059.46429446
ACGTCTG151200.058.98127415
CAGTCAC151450.058.56554427
CACGTCT155050.057.58400714
GTATGCC107400.057.49923745
TTGTAAT153050.057.23145335
AGTCACC156400.056.62399328
TGTAATC104150.056.47685636
TAATCTC96300.056.45953838
CTTGTAA157300.056.08043734
ACACGTC159650.056.0567913
CGTATGC111250.055.0134544
GTCTGAA164350.054.2620617
ATGCCGT104000.054.13774547
CCTTGTA166400.053.3247733
CTGAACT172100.051.9388119
AGCACAC172950.051.86897710
CCAGTCA172950.051.2848326
Produced by FastQC (version 0.11.2)