FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12769929
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG263100.20603090275599809TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT99750.060.2481142
TCGTATG102300.059.75803443
TATGCCG101400.059.53969246
AGTCACG113100.057.65107728
ACGTCTG115600.057.32967415
CACGGCT115700.056.2956931
GTATGCC111300.055.08040245
CGTATGC110800.055.08008644
CACGTCT121450.054.56814
CAGTCAC120250.054.33793327
ACACGTC122600.054.0026213
GCTACAT119250.053.9254835
ATGCCGT112100.053.45631447
GTCACGG126450.051.6736729
GTCTGAA129350.051.28842517
TACATCT123300.049.6921837
GGCTACA131050.049.675434
CTACATC124850.049.60008636
GCCGTCT112900.048.89121649
CATCTCG128700.047.4195639
Produced by FastQC (version 0.11.2)