FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13557614
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63800.045.42177215
AGTCACA67600.043.4303328
CACGTCT69900.041.8035614
CAGTCAC70150.041.65473627
GTCACAT75100.039.1390229
ACACGTC79600.037.0573613
ACATGAG82350.035.5674532
CACATGA84750.034.60106731
GTCTGAA85650.034.35844417
CCAGTCA88000.033.04863726
TGAGCAT87950.032.5576135
AGCACAC95500.031.43134510
TCACATG100800.029.12589530
TCGGAAG101000.029.0373823
CACACGT103000.028.6059612
CTGAACT104550.028.21340819
GAGCACA107750.028.146349
GAGCATC95800.027.98116336
TCTGAAC109500.027.31598318
TCCAGTC112300.026.14327825
Produced by FastQC (version 0.11.2)