FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13815520
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA188560.13648418590107356TruSeq Adapter, Index 25 (97% over 44bp)
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA157330.11387917356711873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57050.054.1829844
TCGTATG58850.052.8774645
TATGCCG59700.052.124648
ACGTCTG66950.047.35536615
AGTCACA68500.046.03233328
CGTATGC68050.045.9316646
GTATGCC68550.045.6469747
CAGTCAC69650.045.22308327
GTCACAC70800.044.48837329
ACTGATA72500.043.7303934
ATGCCGT71650.043.1420549
TATATCT71700.043.06369439
CACGTCT74000.043.03043714
ACACGTC76750.041.71489313
TCACACT78350.040.28942530
TGATATA79950.039.43955236
GTCTGAA81300.039.0816917
GCCGTCT79200.038.37651
CTGATAT83050.038.2167735
ATATATC87700.035.3643738
Produced by FastQC (version 0.11.2)