FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12840540
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40300.049.22376645
CTCGTAT39400.048.77186644
TATGCCG43000.045.49112348
GTCACGT46450.045.00930829
AGTCACG49200.042.8443228
ACGTCCG49000.042.52617332
TCACGTC50650.041.27704630
CGTATGC48900.040.7784846
ACGTCTG52100.040.6575315
CCGCACA51100.040.30576336
CGCACAT50950.040.01799837
GTATGCC51400.039.1978147
CAGTCAC55250.038.33979827
CACGTCT58250.036.48324214
ACACGTC62100.035.05539713
ATGCCGT56200.034.74485449
GTCTGAA66350.032.4454717
GTCCGCA66550.031.36337534
TCTCGTA66300.028.72340643
GCCGTCT62700.028.66688751
Produced by FastQC (version 0.11.2)