FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bw_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bw_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13728719
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT47950.052.23493642
TCGTATG51200.050.6711743
TATGCCG52000.049.49300446
GTCACGT56750.047.60004429
AGTCACG58150.046.6911928
ACGTAGA60150.044.90894332
ACGTCTG62800.043.7833115
CGTATGC59300.043.51694544
CGTAGAG62650.043.17195533
CACGTAG63150.042.8304531
CAGTCAC65950.041.58744427
GTATGCC62500.041.5095245
CACGTCT67350.040.97938514
ATGCCGT67850.037.77858447
TCACGTA74500.036.67573530
ACACGTC77950.035.629813
GTCTGAA80850.034.34979617
GTAGAGA79350.034.2164234
TAGAGAT81800.033.44452735
AGATCTC82350.031.08472438
Produced by FastQC (version 0.11.2)