FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bs_17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bs_17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15312578
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA153970.1005513245385591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG67650.043.2451215
AGTCACA68450.042.23514628
CAGTCAC69150.041.80760227
GTCACAC71350.040.42180629
CACGTCT74650.039.42157714
CACACTT74050.038.80805631
ACACGTC76200.038.48550413
TGAATCG38750.036.2352937
GTCTGAA82200.035.59007317
TCACACT81100.035.5196830
CTTGAAT80950.035.2447935
TTGAATC76650.035.01669336
AATCGCG20050.034.9301739
CTCGTAT19950.034.0673342
CACTTGA86950.033.32818633
TATGCCG23100.032.259146
AGCACAC92350.032.01904310
TCGTATG20450.030.19764543
CCAGTCA98750.029.31083926
CTGAACT105550.027.8800919
Produced by FastQC (version 0.11.2)