FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_ir108_3bs_17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_ir108_3bs_17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14406353
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG90100.048.66642415
CAGTCAC91950.047.3119127
AGTCACC92650.046.99169528
CACGTCT96700.045.5590414
ACACGTC99000.044.7118613
TCGTATG30750.043.755243
TATGCCG33850.043.62096446
CTCGTAT31750.043.13769542
GTCTGAA104200.042.2133317
AGCACAC117600.037.8779410
CCAGTCA116550.037.35547326
ACCAGAT118450.037.01827232
CACACGT124100.035.61424312
CAGATCA123850.035.01437834
TCTGAAC126850.035.0021918
CTGAACT129150.034.16526419
GAGCACA131800.034.1374559
GTATGCC45050.033.5420445
CTCCAGT131300.033.36922524
TCCAGTC131750.033.12444325
Produced by FastQC (version 0.11.2)