FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_c5_1_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_c5_1_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12378719
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT14700.013.1446851
CGGCAAT27650.010.8568451
CCCTACA21250.010.55385410
TCCCTAC23000.010.0509519
CGGAAAT47050.010.0470931
CTACACG18950.010.01369612
CGCGGAT14200.09.9626531
GCGGAAT21500.09.7897331
CGGCATT26400.09.67181
GGGGACT27850.09.5399261
CGGCACT22400.09.3963961
CGCGAAT13350.09.3046651
GCGAAAT29750.09.1626191
GGGAGAT64050.09.1581821
CGGGACT14450.09.0739251
CCTACAC27400.09.06644211
CGGCAAC30550.08.922681
GGAACAT77850.08.864431
GTCGAAT38800.08.8040581
GGCAATC43950.08.7927832
Produced by FastQC (version 0.11.2)