FastQCFastQC Report
Mon 30 Apr 2018
HVHYTBGX5_n01_c0_2_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHYTBGX5_n01_c0_2_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10294787
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT12700.012.4974331
CGCGAAT12300.010.9402231
CTCGAAT37900.010.7426021
GCGAAAT24300.010.6493151
CGAAAAT54550.010.2467691
CGGACAT18550.010.2302321
CGGCAAT23900.010.1057091
AGGGGGG4906.400678E-79.8579364
AAAAATC102850.09.7619932
CCCTACA17000.09.53909510
GGAAAAT82700.09.4708091
CGGGAAT25050.09.3662961
CGGAAAT36500.09.0749671
CGGCAAC26900.08.978681
GCCAGAT32750.08.955181
CGCGTAT7001.3536919E-88.8724011
CGGATAT29400.08.8019851
GGAACAT63300.08.7213341
GAAAAAT95950.08.6664011
CGAGAAT45000.08.6642831
Produced by FastQC (version 0.11.2)