FastQCFastQC Report
Thu 21 Sep 2017
HVHWMBCXY_l02n02_input_ctrl_replicate_3_root_h3k27me3.3520000009e5e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHWMBCXY_l02n02_input_ctrl_replicate_3_root_h3k27me3.3520000009e5e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7268478
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT18150.013.8833810
CTTCCGA20050.012.1189019
GTGTGCT21600.011.8749512
AAGAGTG17100.011.8449945
GTGCTCT22350.011.2751214
TGTGCTC22650.010.9271073
TGCTCTT24100.010.8295345
TCCGATC26750.010.00868511
AAAGAGT21700.09.95634245
CGTGTGC28000.09.64281751
GCTCTTC29000.09.3873116
TCTTCCG28350.09.1263828
GTAGGGA79300.08.0599745
TAGGGAA65100.07.985815545
GTGTAGG120150.07.9607445
AGAGTGT10800.07.918595345
TGTAGGG94300.07.899591445
GAAAGAG42800.07.677103545
GGAAAGA51200.07.29669945
CCGATCT37150.07.26735312
Produced by FastQC (version 0.11.2)