FastQCFastQC Report
Thu 21 Sep 2017
HVHWMBCXY_l02n01_chip_ctrl_replicate_3_root_h3k27me3.3510000009e58c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHWMBCXY_l02n01_chip_ctrl_replicate_3_root_h3k27me3.3510000009e58c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219370
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG14450.08.09706345
TCTGAAC36850.07.876698545
GTCTGAA42700.07.851459545
GAACTCC25050.07.81454245
TGAACTC29250.07.307852345
CGTCTGA49050.06.92675745
CTGAACT32600.06.901991445
CTCCAGT11600.06.78894145
AACTCCA22300.06.659338545
ACGTCTG56100.06.25682145
CACGTCT65300.06.098908445
ACACGTC73400.05.425739344
AGCACAC109450.05.221676345
CACACGT83800.05.128393745