FastQCFastQC Report
Thu 21 Sep 2017
HVHWMBCXY_l02n01_chip_ctrl_replicate_1_root_h3k27me3.3510000009e53c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHWMBCXY_l02n01_chip_ctrl_replicate_1_root_h3k27me3.3510000009e53c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670539
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCCA14500.012.568933545
TGAACTC24600.010.3353445
CTGAACT29000.09.85342345
GAACTCC19200.09.37480244
TCTGAAC34700.09.07778745
GTCTGAA42300.09.04253145
CTCCAGT7250.08.68963345
ACTCCAG10600.08.49054445
CGTCTGA51600.08.284862545
ACGTCTG60800.07.06810644
TATCAAC4250.0017862396.3534012
CACGTCT70600.06.27831345
ACACGTC80550.06.14523645
CACACGT92600.05.85581745
GTAGTTG5600.00106476225.62688261
TCCAGTC8102.9561126E-55.277764345
CTTGCAC6600.00150082395.11534741
GCACACG106600.05.10777244