FastQCFastQC Report
Thu 21 Sep 2017
HVHWMBCXY_l01n01_input_ctrl_replicate_1_shoot_h3k27me3.3510000009e599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHWMBCXY_l01n01_input_ctrl_replicate_1_shoot_h3k27me3.3510000009e599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433279
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA5109.973519E-98.38217744
GTCTGAA6204.0667146E-87.25791245
AAGAGCA19300.06.761516645
CGAGAAC3400.0096909676.61773930
ACGTCTG6609.214855E-76.47713743
GAAGAGC20050.06.39637544
ACACGTC7955.738093E-75.943271641
CACACGT9955.7516445E-95.87927445
CGGAAGA22100.05.599430642
GCACACG10907.732524E-95.57327844
GAGCACA12652.6557245E-105.51371842
TCGGAAG22500.05.39988741
GGAAGAG23850.05.37724643
CACGTCT8409.478748E-65.357030442
TCTGAAC6150.00347226115.12184443