FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39837469
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2352840.5906098100760367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT15250.028.52043728
CGACGAA17100.026.65053228
GACGATA15700.026.58229829
CGAGTAG25100.022.9805663
AGGCCGA36400.022.88364611
CCGAGTA24900.022.8700662
TTTGGGA90700.022.6190995
AGGCTGA119200.022.4392611
CGCTTGA24800.022.24431629
TCTTATA58450.022.108256
GTAGCTG96300.022.0878226
GATTACA137300.022.06742114
CGCTGCC52600.022.03672822
CTGGGAT141750.021.78142510
AGTAGCT98700.021.7099085
ATTACAG136000.021.65532515
TACAGGC113000.021.6345817
TTACAGG127800.021.58127616
GACGAAG20450.021.50260229
CCCGAGT27750.021.4566841
Produced by FastQC (version 0.11.5)