FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_NT3rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_NT3rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22582024
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTC1175020.520334226905436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC128150.031.3616530
CGATACT128350.031.2881331
GACGATA128900.031.16710929
CGACGAT129500.030.99854728
CCGACGA132300.030.3420227
TGCCGAC135100.029.83014525
GCCGACG137350.029.2837626
TGACGCT138500.029.1089519
CTGCCGA140150.028.82300424
TCTGACG140550.028.75242217
ACGCTGC142400.028.32120321
CTGACGC146750.027.58170518
GATACTC146400.027.52926432
GACGCTG146900.027.49896420
ATCTGAC157000.025.87223816
TATACAC158900.025.5653159
CTTATAC158750.025.4799617
GAATCGC73200.025.22186725
TACACAT163700.024.81156511
TTATACA163450.024.766378
Produced by FastQC (version 0.11.5)