FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_NT3rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_NT3rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20746298
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGC625150.30133086876511656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT65900.030.80925228
GACGATA66750.030.4406729
ACGATAT67950.029.9031630
CCGACGA69850.029.0892727
CGATATG71850.028.32469731
TCTGACG73150.028.14797617
TGACGCT72950.028.1374219
TGCCGAC72450.028.08795525
GCCGACG76650.026.65427826
CTGCCGA76900.026.54502724
ACGCTGC79750.025.91710321
GATATGC78650.025.91639332
CTTATAC83050.024.929847
GACGCTG83700.024.73361220
CTGACGC84450.024.53325318
GAATCGC52800.023.96701825
TATACAC87250.023.8018689
CGAGTAG78450.023.7461813
ATCTGAC88750.023.68676416
CCGAGTA79350.023.606352
Produced by FastQC (version 0.11.5)