FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_NT2rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_NT2rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25543458
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGC1237820.48459374607776284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA129200.031.09474229
CGACGAT129950.030.84158928
ACGATAT130500.030.73588230
CCGACGA133550.030.08150727
CGATATG134000.029.87390931
TGCCGAC136900.029.41448225
TGACGCT139300.029.02306419
TCTGACG141500.028.66255617
GCCGACG140800.028.57762526
CTGCCGA142900.028.21214924
GATATGC142750.028.087732
ACGCTGC147250.027.50860621
CTGACGC149650.027.10184718
GACGCTG153350.026.37433820
TATACAC162100.025.0308599
ATCTGAC163250.024.99076516
GAATCGC74750.024.78442425
CTTATAC165450.024.5248077
AGGCCGA129300.024.39775511
TACACAT169200.024.1374811
Produced by FastQC (version 0.11.5)