FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_NT2rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_NT2rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23180423
Sequences flagged as poor quality0
Sequence length38
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACTCCT1576360.6800393590746813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTC165600.030.5692830
GACGACT165900.030.5332929
CCGACGA167350.030.2015527
TGCCGAC168400.030.05892825
CGACGAC170000.029.81604428
TGACGCT172400.029.64943719
TCTGACG174150.029.38830617
GCCGACG177200.028.5853326
CTGCCGA178150.028.4848124
ACGCTGC181700.028.2093621
CTTATAC183350.028.003847
TATACAC184700.027.8755579
ATCTGAC184450.027.86852516
CGACTCC183100.027.7265931
TTATACA185900.027.6796598
CTGACGC187250.027.3839218
GACGCTG188450.027.20901720
TACACAT190050.027.16244911
TCTTATA192450.026.6963636
ATACACA200700.025.71423510
Produced by FastQC (version 0.11.5)