FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_NT1rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_NT1rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28029798
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG548450.19566676862958482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA60750.030.5507128
CCGACGA64250.028.91085627
TGCCGAC66800.027.78188325
GACGAAG69150.026.9777629
GCCGACG69550.026.77592826
TGACGCT69850.026.75168619
TCTGACG69700.026.7179717
ACGAAGA71750.026.02246930
CTGCCGA72400.025.72038724
CGAAGAG73350.025.43315931
GAATCGC88750.025.00277925
AGGCCGA144400.024.63696911
ACGCTGC77550.024.38269621
CTGACGC78750.024.03356618
AATCGCT94400.023.9304826
TTGGGAG532250.023.88776
AGGCTGA539500.023.62234711
CGAGTAG120200.023.6207943
ATCGCTT112050.023.61704327
AGAATCG96450.023.51998724
Produced by FastQC (version 0.11.5)